Objective To explore the diagnostic efficacy of T2WI combined with DWI and DCE-MRI for detecting prostate cancer using 3.0 T MR scanner based on prostate imaging reporting and data system version 2(PI-RADS V2). Methods A total of 123 patients with pathologically confirmed prostate diseases were included for this retrospective study and all ceses were scanned with traditional MRI(T2WI) and functional MRI(DWI and DCE) before biopsy. The diagnostic efficacy of T2WI, T2WI+DWI, T2WI+DCE, T2WI+DWI+DCE compared with pathological results were analyzed according to PIRADS V2. Results Of the 123 prostate diseases patients, 72 were with prostate cancer and 51 with non-prostate cancer. The AUC of T2WI, T2WI+DWI, T2WI+DCE, T2WI+DWI+DCE for predicting prostate cancer based on PI-RADS score was 0.911, 0.936, 0.935, 0.962, respectively. Significant differences of AUC were found between T2WI and T2WI+DWI(P=0.010). T2WI and T2WI+ DCE(P=0.016), T2WI and T2WI+DWI+DCE(P=0.001), T2WI+DWI and T2WI+DWI+DCE(P=0.015), T2WI+ DCE and T2WI+DWI+DCE(P=0.012),but not between T2WI+DWI and T2WI+ DCE(P=0.870) through MedCalc software and the best cut-off point was PI-RADS 3 of each sequence. The sensitivity and specificity of T2WI, T2WI+DWI, T2WI+DCE, T2WI+DWI+DCE for predicting prostate cancer were 88.9% and 86.3%, 90.3% and 88.2%,88.9% and 88.2%,93.1% and 92.2%, respectively. Conclusion The diagnostic efficacy of 3.0T MR scanner for prostate cancer detection between sequences based on PI-RADS V2 were different and the combination of T2WI+DWI+DCE with the highest diagnostic value can be as the best combination approach.