Objective To summarize and discuss the application effect of nursing safety intervention mechanism in the safety management of operation room. Methods During the period January 2014-2015 year in June 500 cases of surgical patients in the operation room in our hospital as the control group subjects. The application of nursing safety intervention mechanism of operation room in our hospital in July 2014, and July 2015-2016 year in June during the 500 cases of surgical patients in the operation room in our hospital as the research objects of the observation group, the nursing effects were compared between the two groups. Results In the observation group, the incidence of medical errors in 0.20% was significantly lower in the observation group the incidence of medical errors 1.60%, a statistically significant difference between the groups (P < 0.05); the satisfaction rate of the observation group (96.5±2.2) were significantly higher than the control group of nursing satisfaction score (85.6±3.5), the difference was statistically significant (P<0.05). Conclusion The nursing safety intervention mechanism is effective in operation room safety management, which can significantly reduce the medical errors and improve the satisfaction degree of nursing service, and is worthy of popularization.