Objective To analyze the clinical and CT manifestations of neonatal cerebral infarction, and to improve the level of diagnosis and differential diagnosis of neonatal cerebral infarction. Methods Retrospective analyze the clinical and CT data of 27 cases with neonatal cerebral infarction. Results There were 15 cases of cerebral infarction located on the left hemicerebrum (55.6%); 6 cases located on the right hemicerebrum (22.2%); 6 cases located on bilateral hemicerebrum (22.2%). 20 lesions were in occipital lobe (74.1%); Middle cerebral arteries were involved in 25 lesions (92.6%). In the CT images, the lesions were shown as low density wedge or sector shapes with bottom outlying, with gray matter affected at the same time. The edges of the lesions were clear, with the brain ditch shoaled or disappeared. The peripheral edema was lighter. The midline structures were slightly shifted. Conclusion The clinical manifestations of neonatal cerebral infarction were lack of specificity, and CT examination is an important method for diagnosis of neonatal cerebral infarction.