Objective To investigate application value of spiral CT perfusion technology combined with biochemical indexes in the diagnosis and condition evaluation of acute pancreatitis (AP). Methods The clinical data of 84 AP patients admitted from January 2014 to June 2017 in our hospital were retrospectively analyzed. The patients were divided into 5 groups according to the Balthazar classification. Group 1 consisted of 17 patients, 16 patients in Group 2, 18 patients in Group 3, 17 patients Group 4 and 16 patients Group 5. The perfusion parameters were obtained by spiral CT scan, and the biochemical indexes were detected by ELISA and rate assay. Results BF and BV decreased gradually with the progress of AP, and the BF and BV in Group 1, Group 2, Group 3, Group 4 and Group 5 were significantly different, with statistical significance (P<0.05). There was no significant change in MTT and PS, and the MTT and PS in Group 1, Group 2, Group 3, Group 4 and Group 5 had no significant difference (P>0.05). The levels of S-Amy, CRP, U-Amy and HCT in Group 1, 2, 3, 4 and 5 were significantly different, with statistical significance (P<0.05). The results showed that the correlation between S-Amy, U-Amy, CRP and BF, BV, MTT and PS was not statistically significant (P>0.05). HCT was negatively correlated with BF and BV, with statistical significance (P<0.05), but had no significant correlation with MTT and PS (P>0.05). Conclusion In early-phase AP, the CT perfusion parameters of BF and BV decreased with the development of the disease, and the biochemical indexes of S-Amy and U-Amy increased. While S-Amy and U-Amy decreased in severe AP, suggesting that there is a negative correlation between HCT and BF and BV, which is an important indicator of pancreatic microcirculation and is conducive to prognosis.