Objective To analyze the application values of digital subtraction angiography (DSA) and brain CT in the diagnosis and treatment of patients with cerebral infarction. Methods 80 cases of patients with cerebral infarction treated in our hospital from January 2016 to January 2017 were selected for the study and divided into the A group (n=40) and the B group (n=40) according to different examination methods. The A group was given the DSA, and the B group was given the craniocerebral CT, and they were treated according to the test results. The vascular image quality, lesion detection and short-term neurological deficit (NIHSS) score were compared between the two groups, and the application effect of DSA in the treatment of cerebral infarction was observed. Results There was no significant difference between the two groups in intracranial vascular image quality grade and the scores of basilar artery and posterior communicating artery image (P>0.05). The scores of internal carotid artery petrous segment and internal carotid artery siphon segment, and NIHSS score after 24h of treatment in the A group were significantly lower than those in the B group (P<0.05). There was no significant difference in the detection rate of infarction > 2mm between the two groups (P>0.05). The detection rate of infarction < 2mm in the A group was significantly higher than that in the B group (25% vs 7.50%) (P<0.05). Among the 40 patients ib the A group, there were 12 cases (30%) with end occlusion of right internal carotid artery by the DSA, and they were recanalized after thrombectomy and there was no residual stenosis. And there were 14 cases (35%) with right frontotemporal and basal ganglia cerebral infarction. And there were 9 cases (22.50%) with artery M1 segment occlusion in right cerebra and recanalized after thrombolytic therapy. And there were 3 cases (7.50%) with posterior inferior or distal artery occlusion of the left vertebral artery cerebellum, and the cerebellum posterior inferior artery was developed well after thrombolysis. Conclusion Compared with brain CT, DSA has better angiography quality, and can have more accurate lesions detection of patients with cerebral infarction, and it can provide an effective reference for clinical treatment.