Objective To explore the correlation of contrast-enhanced ultrasound of breast cancer hemodynamic parameters and VEGF, Flk-1/KDR expression. Methods 95 cases of abnormal breast patients and 25 normal breast volunteers in our hospital were selected from December 2012 to December 2014. They were divided into normal group, simple hyperplasia group, atypical hyperplasia group, intraductal carcinoma in situ and invasive ductal carcinoma group. The expression of MVD and VEGF and Flk-1/KDR were measured. Results There were significant differences in peak intensity, area under the curve and clearance time in each group (P<0.001). The MVD values of each group were (18±5), (20±5), (30±4), (31±8), (53±4), there was significant difference between different group (P<0.001). As the patient's condition worsened, the MVD of the breast increased, and the value of VEGF and Flk-1/KDR increased from the normal group to the invasive ductal carcinoma group, and there was a significant difference (P<0.001). Conclusion The progression of breast cancer is associated with cardiovascular formation and expression of VEGF and Flk-1/KDR receptors. By monitoring the density of blood vessels, contrast-enhanced ultrasound can provide reference for the expression of VEGF and Flk-1/KDR receptors.