Objective To analyze the computed tomography (CT) and magnetic resonance imaging (MRI) manifestations of gallbladder tuberculosis. Methods 300 patients with gallbladder tuberculosis confirmed by clinical pathology were enrolled in the study. All patients were examined by CT and MRI, and their clinical data were retrospectively analyzed. CT and MRI manifestations were summarized. Results The sensitivity, specificity, accuracy and Kappa value of CT were 95.86% (278/290), 80.00% (8/10), 95.33% (286/300) and 0.51, respectively, while those of MRI were 94.83% (275/290), 70% (7/10), 94.00% (282/300) and 0.41, respectively. The sensitivity, specificity, accuracy and Kappa value of MRI+CT were 97.93% (284/290), 80.00% (8/10), 97.33% (292/300) and 0.65, respectively. The CT manifestations included thickening of the gallbladder wall, uniform density in the gallbladder, and clear boundary with the adjacent liver. Contrastenhanced scanning showed slight enhancement of the gallbladder wall, thickening of the gallbladder wall, uneven density in the gallbladder, unclear boundary with the adjacent liver, inhomogeneous enhancement of the gallbladder wall and separated enhancement shadow. MRI manifestations included oval abnormal signals in the gallbladder area, short T1 and long T2 signals in the center of the tuberculosis, lesions surrounded by short T2 and short T1 signals, and the outer layer showing semi-circular short T1 and long T2 signals. Conclusion CT and MRI manifestations of gallbladder tuberculosis are typical. The diagnostic accuracy rates of CT and MRI are high. They can provide important guidance for diagnosis and treatment and evaluation of the prognosis, combined with the clinical data.