Objective To optimize the HR-MRI sequences of carotid arteries in Bama minipigs and to repeated measurement test. To lay the technical foundation for the qualitative and quantitative study of carotid lesion in minipigs. Methods The carotid arteries of three healthy Bama minipigs were examined by 3.0T MRI system, and the optimized high-resolution sequences included 2D FSE T2WI, T1WI and contrast enhancement. The area of lumen (LA), area of total vessel (TA) and area of wall (WA) of bilateral carotid arteries were measured or calculated repeatedly. Coefficient of variation (CV) and Intraclass correlation coefficient (ICC) were used to test the inter-observer and intra-observer agreement. Results ALL the LA, TA and WA of bilateral carotid arteries expressed good inter-observer agreement: all CVs were less than 15%, all ICCs were greater than 0.4, the ICCs of LA and TA in all sequences were greater than 0.90, and the ICCs of LA, TA and WA in T1WI and enhanced T1WI were greater than 0.75, the differences between two observers had no significance(P>0.05). Similarly, the intraobserver agreement was excellent: all CVs were less than 15%, all ICCs were greater than 0.4, the ICCs of LA and TA were greater than 0.95, the differences between twice measurements by one observer had no significance(P>0.05). Condlusion The optimized HR-MRI sequences in this study can improve the image quality, and the sequences could be used in the quantitative study of carotid morphology in Bama minipigs.