Objective To explore the study value of multi-slice CT angiography(MSCTA) in the anatomical structure of basicranial and upper cervical vertebrae pedicle screw. Methods 51 patients suspected or conformed upper cervical disease were collected, which perform cervical CTA, choose the artery phase data to perform reconstruction at GEAW4.4, including Volume Rendering(VR), Maximum Intensity Projection(MIP) and Multi-planar Reformation(MPR) to show the courser and anatomy variation of vertebra artery, Measure the pedicle screw data of C1,C2,C3 and occipital in MPR images, including pedicle length(PL), pedicle width(PW),height(PH), pedicle median angle (PMA), pedicle superior angle (PSA), occipital thickness of 1cm, 2cm, 3cm and transverse sinus interchange plane to skull base. SPSS-19.0 statistical packages were used for data entry and statistical analysis and pair sample t-test statistical analysis for the difference between left and right side. Results Both of MIP and VR can observe courser and nearby space relationship of cervical artery. Pair sample t-test statistical analysis for the difference of left and right side: there was marked difference only between two sides of C3-PH. Conclusion MSCTA can reflect the morphology of upper cervical vertebral pedicle screw and skull base, also can provide an accurate reference for imaging anatomy research and clinical surgery.