Objective To investigate the value and differentiation of multislice computed tomography scanning and perfusion for pancreatic carcinoma imaging. Methods The clinical data of 44 patients with pancreatic carcinoma were analyzed retrospectively. All the patients received multislice computed tomography scanning and perfusion. Multislice computed tomography scanning included plain scan and multi-phase scan. The prevalence of pancreatic carcinoma at different time (plain scan phase, arterial phase, portal venous phase, pancreatic phase) was compared. The blood flow(BF), blood volume (BV), permeability surface(PS), mean transit time (MTT) were measured and compared between the pancreatic carcinoma and the normal pancreatic tissue. Results In the multislice computed tomography scanning, the positive rate of pancreatic carcinoma at pancreatic phase was the highest. There was no significantly difference for detection of pancreatic carcinoma between portal venous phase and pancreatic phase. And the positive rate of pancreatic carcinoma at portal venous phase and pancreatic phase were higher than that at plain scan phase and arterial phase. And there was significantly difference. The density value in multi-phase scan between the pancreatic carcinoma and the normal pancreatic tissue was significantly difference(P<0.05). The CT differential value at pancreatic phase was higher than that at arterial phase and portal venous phase(P<0.05). In multislice computed tomography scanning, the density value between the pancreatic carcinoma and the normal pancreatic tissue was no difference. In CT perfusion imaging, BF, BV and PS value of pancreatic tumor were less than those in normal pancreatic tissue. While MTT value was more than that of normal pancreatic tissue. There was significantly difference between them. Conclusion Multislice computed tomography perfusion imaging plays an critical role in diagnosis of pancreatic cancer and BF, BV, MTT and PS diagnosis and differential diagnosis of pancreatic tumor were more.