Objective To compare the diagnostic value of Doppler ultrasonography and multi-slice spiral CT (MSCT) in neoplasms in different sites of bladder. Methods 50 cases of patients with bladder neoplasms confirmed by surgery and pathology who were admitted in our hospital between February 2013 and October 2015 were selected as the study objects. Before surgery, all of them underwent MSCT plain scan, enhanced scan and Doppler ultrasonography. The results of surgery and pathology were taken as control to compare the detection rates of different methods in benign and malignant bladder neoplasms and neoplasms in different sites. Results The sensitivity, specificity and accuracy of MSCT in the diagnosis of bladder neoplasms were 93.5%, 75.0% and 92.0%, respectively while those of Doppler ultrasonography were 82.6%, 75.0% and 82.0%, respectively (P>0.05). The detection rates of MSCT delayed phase in neoplasms in bladder neck and trigonum and neoplasms in any site (75.0%, 76.9%) were significantly higher than those of Doppler ultrasonography (47.1%, 56.5%) and MSCT (44.1%, 52.8%) (P<0.05). There was no significant difference in the detection rate of neoplasms in bladder neck, trigonum and other sites between different examination methods(P>0.05). Conclusion The accuracy rates of Doppler ultrasonography and MSCT in the diagnosis of bladder cancer are high. MSCT in detection of neoplasms in bladder neck andtrigonum is superior to Doppler ultrasonography. In clinic, flexible application of MSCT postprocessing technique can improve the detection rate of tumor lesions.