Objective Application of magnetic resonance imaging (MRI) scan in combination with a variety of functional imaging of normal prostate, prostate cancer and prostate hyperplasia patients for biological behaviour, metabolic product characteristics and dynamic enhancement time-signal curve for this paper. Methods Selected age 47-85, 153 cases of elderly men patients in the conventional magnetic resonance imaging. Normal prostate patients combined diffusion weighted image can, 3D1H-MR spectroscopy, Suspected diagnosis of patients with hyperplasia of prostate and joint DWI, MRS and DCE-MRI, DWI and MRS can after analysis, Draw the DCE-MRI mearn curve areas of interest. Statistical analysis of normal prostate biological behavior characteristics, metabolites, Hyperplasia of prostate and prostate two lesions choline (Cho)biological behaviour, metabolites,citrate salt(Cit), characteristics of creatine(Cre) and peak(Cho+Cre)/ Cit the comparison of the mean, Dynamic enhanced nodule hemodynamic characteristics analysis of hyperplastic nodule and cancer,With independent samples t test, P<0.05 think the difference is statistically significant.The MRI diagnosis of prostatic hyperplasia and prostate cancer cases biopsy or cutting biopsy compared the coincidence rate. Results 30 cases of normal prostate central T1WI, or slightly high signal, the peripheral zone T1WI homogeneously middle or slightly low signal,T2WI central moderate signals, Peripheral zone crescent high signal, DWI normal central and peripheral zone of prostate can signal no significant difference. Normal prostate central, prostate hyperplasia, prostate gland area between ADC values difference was statistically significant(P<0.05),Normal prostate peripheral zone and ADC values differences between prostatic hyperplasia has no statistical significance (P>0.05). The hyperplasia and normal prostate peripheral zone lesions metabolites peak than there was no significant difference (P>0.05), prostatic hyperplasia and prostate cancer metabolites peak ratio has significant difference (P<0.05). Conclusion MRI scan T2WI of prostate lesions showed low sensitivity, high specificity, DCE- MRI, DWI and MRS can three inspection methods in the diagnosis of prostate cancer with the characteristic, joint function imaging DWI, MRS, DCE can scan, MRI can largely improve lesions diagnostic sensitivity, specificity, and even the quantitative diagnosis.