Objective To study the application of interventional micro-catheter intubation for the preparation of monkey focal cerebral ischemia model. Methods Ten male Monkeys were anesthetized, the 1.7F micro-catheter were intubated into the middle cerebral artery. Twenty cm (1 mm diameter) autologous thrombus was injected into the middle cerebral artery (MCA). The embolization was confirmed by digital subtraction angiography (DSA). Diffusion Weighted sequence were performed to confirm the occurrence of cerebral infarction, and calculate the ADC value. The brain tissues were examined by routine HE staining after 24 hours of the embolization,. Results After monkey MCA occlusion, the systolic blood was higher (P<0.05). 10 animals were intubated 12 times, the success rate of the intubation in monkey was 100% (10/10). The success rate of intubation in artery was 83.3% (10/12). The mortality rate during the anesthesia and surgery was 0%. One monkey died in 24 hours, the mortality rate in 24 hours was 10% (1/10). ADC values were significantly lower at 2 hours and 24 hours after embolization (P<0.05). ADC values in contralateral normal brain regions were not significantly different between before and after embolization (P>0.05). 24 hours after embolization, the signal intensity of the lesion was increased on the DWI imaging. The brain tissue HE staining showed infarct area of brain parenchyma clear boundary, the brain tissue in the infarct liquefaction, necrosis. High-power microscope showed infarction cell necrosis, such as mesh-like, nuclear fragmentation and dissolution. Conclusion The technique of micro-catheter super selective intubation can be used to prepare a stable monkey model of focal cerebral ischemia.