Objective To investigate the application value of multivoxel proton magnetic resonance spectroscopy (1 H-MRS) and variation of metabolite concentrations and its clinical significance in hyperacute and acute cerebral infarction. Methods 23 cases of hyperacute cerebral infarction and 19 cases of acute cerebral infarction were selected for the study,underwent conventional magnetic resonance (MRI) and 1 H-MRS scans Then to compare the concentrations of Cr, Cho, NAA and other metabolites in the infraction center,the border region, the normal region around the lesion and the contralateral area. Results ①In the hyperacute cerebral infarction, the concentrations of Cr, Cho, NAA in the infraction center,the border region, the normal region around the lesion was significantly lower than the contralateral area (P<0.05), Lac increase compared with the contralateral area. ②In the acute cerebral infarction, the concentrations of Cr, NAA in the infraction center, the border region, the normal region around the lesion was significantly lower than the contralateral area (P<0.05), the concentrations of Cho in the infraction center and the border region was significantly lower than in the contralateral area (P <0.05), Lac increase compared with the contralateral area. There was no significantly different in the regional metabolites between the hyperacute cerebral infarction and the acute cerebral infarction. Conclusion Multivoxel 1 H-MRS can directly, accurately and comprehensively display the metabolic changes of brain tissue in cerebral infarction side and contralateral area,it not only conducive to the study of the pathophysiological changes after cerebral infarction, but also to provide the evidence for clinical treatment of cerebral infarction.