Objective To investigate the diagnosis and prognosis value of DWI in detecting cervical cancer. Methods 100 patients with cervical cancer were selected from January 2011 to January 2014. 100 cases of cervical cancer radiotherapy troubles and 100 cases of healthy patients were also closed.The apparent diffusion coefficient were analyzed. Results Compared with normal cervical glands, the apparent diffusion coefficient values of cervical fibrous was 1.70±0.45×10-3mm2 /s, after comparative analysis, the difference was statistically significant (t=6.834,P<0.05). 100 cases of cervical lesions in DWI showed high signal state. According to the depth of patient invasion, the low signal of cervical fiber disappeared. Compared with normal cervix, the apparent diffusion coefficient value of cervical cancer patients was 1.02±0.32×10-3mm2 /s, after comparative analysis, the difference was statistically significant (t=12.412,P<0.05). Compared with patients with cervical cancer, the prognosis of patients with normal apparent diffusion coefficient value was 1.45±0.41×10-3mm2 /s, after comparative analysis, the difference was statistically significant (t=7.395,P<0.05). Compared with newly diagnosed with cervical cancer, cervical cancer recurrence in patients with apparent diffusion coefficient value was 0.82± 0.20×10-3mm2 /s, after comparative analysis, the difference was statistically significant (t= 8.295, P<0.05). Conclusion DWI approach can significantly distinguish normal cervix and cervical disease and it also being able to significantly observe human cervical tissue changes after radiotherapy.