Objective To investigate the application value of 3.0 T magnetic resonance Diffusion Tensor Imaging (DTI) technology in the surgical removal of gliomas. Methods 28 cases of glioma patients were randomly divided into experimental group (conventional MR sequences and sequence of DTI scan) and the control group (conventional MR sequences scanning),the treatment group patients have the application of DTI reconstruction to effectively pyramidal tract(EPT) of cerebral white matter fiber tracts, with the optimal surgical approach, guiding intraoperative excision of tumor and maximum protection EPT; the control group patients have the tumor resection by guiding with the conventional magnetic resonance imaging, both of the groups were performed DTI scan 3 months before operation and 3 months after it. the scan images, the microscopic tumor patients with postoperative morbidity rate, and Kamofsky life status score (KPS) comparative were analyzed. Results (1) the control tumor microscopically the cutting rate was 57.2%. Experimental group 64.2% (P>0.05); (2) the control of postoperative morbidity was 71.4%, the experimental group 21.4% (P<0.05); (3) the prognosis assessment: the control group KPS68.36±10.60. Experimental group KPS 85.43 ±5.65 (P<0.05). Conclusion DTI technology can provide three-dimensional anatomical information between the lesions and EPT for gliomas involving EPT preoperatively, guiding the performer largest radius and the removal of the tumor at the same time, which can protect the EPT and significantly reduce postoperative disability rate and improve patient quality of life after surgery