Objective To prospectively evaluate the effect of intravenous administration of gadoxetic acid disodium (Gd-EOB-DTPA) on diffusion weighted imaging (DWI) of normal rabbit's liver on 3.0-Tesla system. Methods Six health New Zealand rabbits were performed DWI on 3.0-Tesla system before and 20 minutes after injecting Gd-EOBDTPA (0.2ml/kg) through the left ear marginal vein with the same protocol. DWI with b values of 50, 400 and 800s/mm2 , and ADC values of right lateral lobe of liver were analyzed on MR Workstation. The Signal-to-noise ratio (SNR) on DWI and ADC values of the normal liver were measured and compared between these two group data by using the paired-samples t-test. Results ADC values of liver decreased slightly on 20 minutes post-contrast DWI than those of pre-contrast, (0.613±0.274)×10-3mm/ s vs (0.637±0.302)×10-3mm/s), but there was no significant difference (t=0.680, P=0.501) between them. SNRs of the liver parenchyma on DWI with different b values also showed no significant differences between 20 minutes post- and pre-contrast agent administration (P＞0.05). Conclusion ADC values and SNR of normal rabbit's liver on 20 minutes DWI after intravenously injecting Gd-EOB-DTPA had no significant changes compared with pre-contrast DWI.