Objective To compare the diagnostic value of 64-slice CT coronary angiography (CTA) and coronary angiography (CAG) in myocardial bridge (MB) and mural coronary artery (MCA). Methods The imaging manifestations of CTA in 206 cases between December 2009 and January 2011 were retrospectively analyzed, 22 of whom underwent the CAG examination. The number of MB and MCA and the degree of stenosis of the MCA were evaluated. Results Forty-four vascular lesions in 41 patients were found by CTA, including 36 deep MB and 8 shallow MB. Nineteen vascular lesions in 19 patients were observed by CAG while no lesions were detected in two cases (CTA showed shallow MB of LAD). In 41 MB diagnosed by CTA, the degree of MCA stenosis was less than 50%. In 19 MB diagnosed by CAG, the degree of MCA stenosis were less than or equal to 50% in 9 cases, more than 50% and less than or equal to 75% in 6 cases, greater than 75% in 4 cases. Conclusion By application of 64-slice CTA, the MB-MCA can be observed but the observed MCA stenosis observed is different from CAG. CAG can observe dynamically the degree of stenosis of the MCA. Combination of CTA and CAG is the most accurate diagnosis for MB and the stenosis degree of MCA.