Objective The clinical effect of sequential type of OPCABG and non-sequential type of OPCABG was compared by a number of indicators. Methods From 2009 January to 2015 January underwent coronary artery bypass graft operation in our hospital were randomly selected for sequential type of OPCABG and non-sequential type of OPCABG patients all 28 patients as research object, a total of 56 cases. According to different operation modes, the non sequential OPCABG patients were recorded as the control group, and the sequential OPCABG was recorded as the observation group, compared the two groups of anesthesia induced by 5Min after operation (T0), 1d after operation (T1) and 2d (T2) of myocardial troponin I (cTnI) concentration, creatine kinase isoenzyme (CK-MB) activity, the concentration of blood glucose and the number of vessels used in the bridge, operation time, bleeding volume, hospitalization expenses. Results Two groups of patients with cTnI, CK-MB, NT-proBNP on T0 moment, any time the level of IL-6 were no difference (P>0.05), in T1, T2 moments CTnI, CK-MB, NT-proBNP were lower than the control group In addition, the use of the number of the observation group in the bridge, the operation time, blood loss, hospitalization costs were significantly lower than the control group. Conclusion The degree of myocardial injury in the sequential OPCABG is less than the non sequential OPCABG. The use of bridge vessel in sequential OPCABG is less, the operation time is shorter, the cost of hospitalization is lower, especially for the the patients with Aortic atherosclerosis, and the patients with lack of the bridge vessel, which greatly increased the OPCABG surgical indications.