目的 研究介入微导管插管法 制备猴局灶性脑缺血模型技术以及影像 学表现。方法 雄性广西猕猴10只，将 1.7F微导管插管至大脑中动脉M1段注 入自体血栓20cm(直径1mm)，大脑中动 脉正侧位造影确认栓塞成功，栓塞后2 小时MRI扩散加权序列扫描再次确认脑 梗死，并计算ADC值。栓塞后24小时， MRI扫描结束后处死动物取脑组织行Ｈ Ｅ染色观察病理改变。结果 动物大脑 中动脉栓塞后收缩压较栓塞前升高，差 异有统计学意义(P<0.05)。10只动物大 脑中动脉共插管12次，其中两只动物 颈内动脉迂曲插管不成功，将导管换 至对侧大脑中动脉插管成功，动物插 管成功率为100%(10/10)，血管插管成 功率为83.3%(10/12)。麻醉和手术期间 动物死亡率为0，术后24小时以内死亡1 只，死亡率10%(1/10)。栓塞后2小时以 及24小时病灶ADC值降低，核心区ADC值 降低较边缘区明显，差异有统计学意义 (P<0.05)，对侧大脑正常区ADC值在栓塞 前后差异无统计学意义(P>0.05)，栓塞 后2小时病灶在DWI图像上信号升高，24 小时明显升高。24小时后脑组织HE染色 显示脑梗死灶边界清楚，梗死灶内脑组 织液化、坏死，高倍镜下显示细胞坏死 如筛网状，细胞核碎裂和溶解。结论 介 入微导管超选择插管技术能制备稳定的 猴局灶性脑缺血模型。

Objective To study the application of interventional micro-catheter intubation for the preparation of monkey focal cerebral ischemia model. Methods Ten male Monkeys were anesthetized, the 1.7F micro-catheter were intubated into the middle cerebral artery. Twenty cm (1 mm diameter) autologous thrombus was injected into the middle cerebral artery (MCA). The embolization was confirmed by digital subtraction angiography (DSA). Diffusion Weighted sequence were performed to confirm the occurrence of cerebral infarction, and calculate the ADC value. The brain tissues were examined by routine HE staining after 24 hours of the embolization,. Results After monkey MCA occlusion, the systolic blood was higher (P<0.05). 10 animals were intubated 12 times, the success rate of the intubation in monkey was 100% (10/10). The success rate of intubation in artery was 83.3% (10/12). The mortality rate during the anesthesia and surgery was 0%. One monkey died in 24 hours, the mortality rate in 24 hours was 10% (1/10). ADC values were significantly lower at 2 hours and 24 hours after embolization (P<0.05). ADC values in contralateral normal brain regions were not significantly different between before and after embolization (P>0.05). 24 hours after embolization, the signal intensity of the lesion was increased on the DWI imaging. The brain tissue HE staining showed infarct area of brain parenchyma clear boundary, the brain tissue in the infarct liquefaction, necrosis. High-power microscope showed infarction cell necrosis, such as mesh-like, nuclear fragmentation and dissolution. Conclusion The technique of micro-catheter super selective intubation can be used to prepare a stable monkey model of focal cerebral ischemia.