Objective To explore the consistency and difference of ultrasound and multislice spiral CT (MSCT) in the diagnosis of thyroid nodule calcification. Methods The clinical data of 364 cases of patients with thyroid nodules treated with surgery in our hospital from October 2014 to October 2017 were analyzed retrospectively. All patients were given preoperative ultrasound and MSCT examinations, and given postoperative pathological examination, and there was at least 1 test results suggesting the presence of calcification. The detection rates of thyroid nodule calcification by ultrasound and MSCT were compared, and the consistency between the results of the two examinations and the postoperative pathological findings was analyzed, and the imaging features of thyroid nodule by ultrasound and MSCT were analyzed. Results The calcification detection rates of malignant thyroid nodules and benign nodules such as papillary carcinoma and papillary micro-carcinoma by ultrasound were higher than those by MSCT(P<0.05). And the consistency of ultrasound and pathology was 88.68% (47/53), which was higher than that of MSCT and pathology with 73.58% (39/53) (P<0.05). Conclusion The accuracy of ultrasound in the diagnosis of thyroid nodule calcification is higher than that of MSCT, and the consistency is higher in ultrasound and pathology.