Objective To investigate the clinical values of multi-slice CT in the diagnosis of of asteatotic angiomyolipoma of kidney and non-clear cell renal cell carcinoma. Methods Eight-five patients with non-clear cell renal cell carcinoma (RCC group) and 42 patients with angiomyolipoma of kidney (lipoma group) were selected as the research object, recorded and analyzed the characteristics of the multi-slice spiral CT routine scan and enhanced scan in the two groups. Results The maximum diameter of the RCC group and the lipoma group were (72.34±21.48)mm and (43.67±15.20)mm respectively, and the RCC group was significantly higher than that in the lipoma group(P<0.05). The incidence of alcification, cup mouth sign, blurred boundary, false envelope, delayed enhancement, and renal metastasis in the RCC group were significantly higher than that of the lipoma group(P<0.05). The CT values of the RCC group was (40.82±2.91)Hu(plain phase), (74.29±17.30)Hu(cortical phase) and (76.20±13.30)Hu(parenchymal phase), lower than the lipoma group of (50.93±2.78)Hu, (123.95±17.20)Hu and (114.20±5.20)Hu respectively(P<0.05). Conclusion The clinical features of angiomyolipoma of kidney and non-clear cell renal cell carcinoma are similar, they can be differentiated by the maximum tumor diameter, CT features and enhanced CT value, which is worth popularization and application.