Objective To investigate the clinical application value of ultrasound and MRI in the screening of ovarian lesions. Methods The clinical, pathological, ultrasonic and MRI data of 39 patients with ovarian lesions confirmed by pathology and surgery who were admitted into our hospital between January 2015 and January 2016 were retrospectively analyzed. Results Before surgery, 14 cases underwent ultrasound examination and 25 cases underwent MRI scan. The diagnostic accuracy of ultrasound was 85.71% while of MRI was 88.00% (P＞0.05). Ultrasound contrast showed that there were oval cystic and solid mixed masses in ovaries of patients with ovarian serous papillary cystadenocarcinoma, without internal echo or with punctate echoes, with blood flow signals and pelvic effusion. Left and right side ovarian serous papillary cystadenocarcinoma showed that there were solid masses in right ovary, with strong echo and weak blood flow signal. MRI data showed that there were oval cystic and solid mixed masses in ovaries of patients with ovarian serous papillary cystadenocarcinoma, with fuzzy boundaries, showing mixed signal and dynamic enhanced scan showed inhomogenous cystic wall and localized obvious enhancement in solid parts; In ovaries of patients with left and right side fallopian tube serous cystadenocarcinoma, there were oval or circular solid masses. T1WI showed low signal or low and equal signal, and D2WI showed inhomogeneous high signal. Dynamic enhanced scan showed that there was obvious enhancement in cystic wall, intracapsular patchy shadows and solid parts. Conclusion The accuracy rates of ultrasound and MRI in diagnosis of ovarian lesions are high but still need to be improved; The findings of the two imaging methods has certain characteristics. All can clearly show the characteristics of lesions, which is of important clinical value in screening and diagnosis of ovarian lesions.