Objective To investigate the correlation of 1 H-MRS, ADC value with mutation of p53 gene in brain gliomas and peritumoral edema region, which could improve application values. Methods Between September 2011 and December 2015, the MR scanning, 1 H-MRS, and DWI were conventionally performed before the operations in 30 patients with gliomas diagnosed by the postoperative pathological examination. Postoperative mutation of p53 gene was detected in parenchyma region. N-acetyl aspartic acid (NAA), choline (Cho), creatine (Cr), which were main metabolic products in the glioma parenchyma and peritumoral edema region, The correlativity of Cho/NAA、 Cho/Cr、NAA/Cr and ADC with P53 expression in tumor was analyzed. Results (1) The Cho/NAA ratio was positively correlated with the mutation of p53 gene in brain gliomas and peritumoral edema region (r=0.621, P=0.012, r=0.421, P=0.022), the Cho/ Cr ratio was positively correlated with the mutation of p53 gene in brain gliomas(r=0.901, P=0.019). (2)The Cho/Cr ratio was not correlated with the mutation of p53 gene in peritumoral edema region(P=0.431). The NAA/Cr ratio was not correlated with the mutation of p53 gene (P=0.912,P=0.742) in brain gliomas and peritumoral edema region. (3)The ADC values was negatively correlated with mutation of p53 gene(r=-0.792, P=0.014). The ADC values was not correlated with mutation of p53 gene(P=0.291) in peritumoral edema region. Conclusion DWI examination and 1 H-MRS can effectively show the mutation of p53 gene in the glioma, especially Cho/NAA ratio is highest, these indicators could exactly evaluate mutation of p53 gene forglioma.