Objective To explore the clinical value of ultrasonic cardiogram (UCG) combined with CT angiography (CTA) in the diagnosis of mitral valve prolapse (MVP). Methods The clinical data of 28 patients with MVP were retrospectively analyzed retrospectively. The differences of lesions condition were compared by UCG, CTA and surgical measurement. The surgical findings were used as the gold standard to analyze the diagnostic efficacy of preoperative UCG, CTA and the combination of the two. Results There were no significant differences in the annulus circumference, valve leaflet thickness and choraetendinae rupture rate by UCG, CTA and surgical measurement (P>0.05), and the annulus displacement level measured by UCG was significantly lower than that by surgical measurement, and the area of tentorial region measured by CTA was significantly lower than that by surgical measurement (P<0.05). The accuracy rate by simple UCG and simple CTA was significantly lower than that by UCG combined with CTA in the diagnosis of MVP (P<0.05). Conclusion Both UCG and CTA can obtain rich and accurate MVP lesion information. The combination of these two methods helps to improve the diagnostic accuracy of the diseaseThe combination of the two can complement each other to enhance the diagnostic efficacy, and it has high clinical application value.