Objective To analyze the clinical value of magnetic resonance cholangiopancreatography (MRCP) in the diagnosis of choledocholithiasis and the influencing factors of psychological status. Methods A total of 55 patients with choledocholithiasis in the hospital from March 2018 to January 2019 were enrolled. All patients underwent two-dimensional and three-dimensional MRCP (2DMRCP, 3DMRCP) and T2-weighted imaging fat suppression sequence (FIESTA), and they were confirmed by the results of intraoperative lithotomy and cholangioscopic exploration. After examination, the psychological status was evaluated by the Symptom Checklist 90 (SCL90). The general data and the relationship between the MRCP examination and the total score of SCL-90 were analyzed. Logistic regression analysis was performed. Results The display rates of the three scan methods for examining the stones equaled to or were greater than 80%. The combined diagnosis rate was 94.55%. There were only 3 cases not detected due to stone diameter less than 5mm. The shape of the stones was round, elliptical, strip and irregular. In the images of 2DMRCP, 3DMRCP and FIESTA , choledocholithiasis showed low or slightly low signal and filling defect shadow. The SCL-90 evaluation for the 55 patients showed that the scores in depression, anxiety, horror and paranoia were greater than 2 points. The scores of the other dimensions were basically normal. The total score of SCL-90 was (26.23±5.74) points. Univariate and multivariate analysis showed that age, no psychological intervention before examination, long duration of MRCP and complications were risk factors affecting the psychological status of patients undergoing MRCP (P<0.05). Conclusion The diagnostic value of 2DMRCP, 3DMRCP combined with FIESTA examination is high for choledocholithiasis. But MRCP examination can affect psychological status of patients. Intervention should be conducted from the aspects such psychological counseling, shortening examination time and controlling complications.