Objective To compare the parameters of MSCT perfusion weighted imaging of glioma with different grades and their correlation with VEGF expression. Methods 53 patients with glioma admitted to our hospital from June 2017 to April 2018 were enrolled. All patients underwent MSCT perfusion imaging. The laboratory data and imaging data were collected.w associate chief physician summarized the imaging features of glioma in CT and MRI, and compared the parameters of MSCT perfusion weighted imaging and their correlation with expression of VEGF. Results In MSCT plain scan, glioma mainly showed uniform or uneven low-density lesions, a few were mixed lesions, and a few patients showed shadow of calcification. In the MSCT enhanced scan, there may be different degrees of low density areas with cystic liquefaction and necrosis. In perfusion weighted imaging, the perfusion of the solid area of tumor in most patients was higher than that of the same position of contralateral side,and it appeared as zone of perfusion with increasing brightness when the amount of perfusion increased.There were significant differences in rCBV values of gliomas with different grades.The rCBV values of grade IV were significantly higher than those of grades I, gradesII and grades III, the difference of data was statistically significant (P<0.05). There were significant differences in VEGF expression levels of gliomas with different grades. As grade of glioma increased, VEGF expression levels increased gradually (P<0.001). There was a positive correlation between rCBV values and VEGF expression levels in glioma of different grades (r=0.763, P<0.001). Conclusion There was a positive correlation between rCBV value and VEGF value in parameters of MSCT perfusion weighted imaging.As the grade of glioma increases, the rCBV value and VEGF value gradually increase.