Objective To detect the tumor markers expression of Her2 and uPA of urine in patients of Ureteral carcinoma (upper tract urothelial carcinoma, UTUC) and study the CT images of UTUC, to improve the understanding of ureteral carcinoma pathogenesis and cognition as well as the diagnosis accuracy and specific degrees, providing certain guiding significance with early diagnosis and treatment for ureteral carcinoma. Methods From January 2011 to now, 22 patients with UTUC (diagnosed by pathological examination)and 20 patients with Benign ureteral diseases were studied, retrospective analysis was applied for all the data including the resultsof CT and Her2 and uPA. Comprehensive assessments were used to study the diagnose effect of united use of CT and Her2 and uPA. Using enzyme-linked immunosorbent experiment (ELISA) test, to test 22 cases of UTUC and 20 cases of Benign ureteral diseases of Her2 and uPA content in the urine. Results Her2 and uPA content in the urine of patients with ureteral carcinoma patients with benign ureteral diseases have increased significantly. The diagnosis accuracy of CT for UTUC is 84.6%, which specificity is 70.8%; The diagnosis accuracy of Her2 and uPA is 70%, which specificity is 69.7%; The combined diagnosis accuracy is 100%, specificity is 95.3%. Conclusion CT combined with Her2 and uPA to diagnosis, can improve the diagnosis accuracy and specificity of UTUC, for early and accurate diagnosis, preoperative formulation of proper operation method, has important guiding significance.