Objective To explore the application value of 3.0T MRI dynamic contrast enhanced scan combined with DWI in the diagnosis of benign and malignant breast lesions. Methods The data of 55 patients with breast lesions treated in our hospital were retrospectively analyzed. All patients underwent 3.0T MRI dynamic contrast enhanced scan combined with DWI. Based on the results of surgery and pathology, the MRI imaging features, morphology and enhancement characteristics were compared between patients with benign and malignant lesions. The accurate rate of 3.0T MRI dynamic contrast enhanced scan combined with DWI in the diagnosis of benign and malignant breast lesions was calculated. Results In patients with benign lesions, the proportion of patients with clear boundaries, regular shape and homogeneous enhancement was significant higher than that in patients with malignant lesions (P<0.05). In patients with benign lesions, there were 11 cases of type I, 26 cases of type II and 1 cases of type Ⅲ, and the proportion of (type I + II) was 97.4% (37/38). In patients with malignant lesions, there was none cases of type I, 4 cases of type II and 13 cases of type III, and the proportion of (type I + II) was 23.5% (4/17) (P<0.05). Conclusion The clinical application of 3.0T MRI dynamic contrast enhanced combined with DWI scan in the diagnosis of benign and malignant breast lesions can achieve effective complementation, which is beneficial to improve the accuracy of diagnosis, and achieve the effective identification of benign and malignant lesions.