Objective To study the value of the One-stop cardiac inspection by 64-slice CT combined with BNP and hs-cTnT in acute coronary syndromes patient assessment. Methods 59 patients with ACS and 11 healthy volunteers were selected in this study, who underwent 64-slice CT One-stop cardiac inspection, and the CT data of coronary stenosis and left ventricular ejection fraction (LVEF), the level of BNP and hs-cTnT, the coronary stenosis, the value of LVEF, the level of BNP and hs-cTnT, and variable heart function were compared and analyzed. Results Count of coronary artery lesion is inversely proportional with degree of stenosis and LVEF. Left anterior descending and left circumflex branch and right coronary artery for diminishing the influence degree of the left ventricle LVEF into an inverse. Moreover, Cardiac function I, II, III and IV patients' concentration of BNP and hs-cTnT level were obviously higher than volunteers. Differences were statistically significant(P<0.01). Different level of cardiac function in patients with hs-cTnT, BNP was a negative correlation with LVEF (r=-0.953, -0.832, -0.943, P<0.01). Conclusion 64-slice CT One-stop cardiac inspection processed in patients with ACS, we could not only judge the stenosis of coronary artery, but also analysis the left ventricular function. LVEF combined with the plasma BNP & hs-cTnT detection can provide the quantitative analysis of heart function damage causing by the ACS. So as to provide objective index to clinical diagnosis and treatment.