Objective To provide imaging bases for clinical diagnosis of cholangiocarcinoma through analyzing multi-phase CT enhanced scan characteristics of cholangiocarcinoma. Methods A retrospective analysis of CT imaging data of cholangiocarcinoma confirmed by pathology was carried out in 70 cases in our hospital from January 2014 to May 2016, enhancement scanning findings of CT scan, multiple phase (arterial phase, venous phase and delayed phase) were observed. Results CT scan showed that there were 70 cases of single cholangiocarcinoma, mostly in left lobe of liver (64.29%), average lesion diameter was 6.5cm. 25.71% were complicated with hepatolith, 20.00% were complicated with left hepatic lobe atrophy. CT scan showed that there were 45 cases of massive type, which accounted for 64.29%, scan findings were low-density shadow, obscure boundary, arterial phase showed that there were no enhancement of lesion in 14 cases, margin mild enhancement in 28 cases, venous phase showed that there were 14 cases of lesion center further reticular enhancement, delayed phase showed that there were 18 cases of striation or inhomogeneous reticular continuous enhancement. 19 cases of cavity growth type, scan showed that there were hepatic portal peripheral soft tissue mass, arterial phase showed that there were hepatic portal peripheral soft tissue mass mild enhancement, venous phase were further enhancement, delayed phase were slow continuous enhancement; 9 cases of infiltrative type, scan showed bile duct wall thickening, lumen stenosis, bile duct wall in the liver were mild enhancement in arterial phase, venous phase were further enhancement, delayed phase were slow continuous enhancement. Conclusion CT scan and multi-phase enhanced scan has a certain specificity in the diagnosis of cholangiocarcinoma, which is conducive to the early correct diagnosis of cholangiocarcinoma.