Objective The MRI manifestations of pelvic floor dysfunction (PFD) and normal female in pelvic anatomy were compared. Methods 40 patients diagnosed PFD in our department from June 2012 to June 2014 were selected, including 20 cases of pelvic organ prolapse, urinary incontinence seven cases, the combined 13 cases. Meanwhile 40 healthy female volunteers at the same age were chosen as the control group. By line static and dynamic MRI scans (MRI) evaluation of the levator ani muscle groups indicators pelvic organ prolapse, the urethral length and support structures around the urethra indicators. Results PFD group shame tail muscle area, static / dynamic measurements iliac tail area and shame rectus muscle area were (2.12±0.43) cm2 / (1.76±0.37) cm2 , (1.96±0.55) cm2 / (1.63±0.48) cm2  and (2.29±0.64) cm2 / (1.87±0.53) cm2 , significantly lower than the normal group (2.69±0.57) cm2 / (2.68±0.49) cm2 , (2.43 ±0.61) cm2 / (2.39±0.40) cm2 and (2.77±0.39) cm2 / (2.81±0.36) cm2 . PFD group levator plate angle, pelvic diaphragm hole diameter, LH line and M-line static / dynamic measurement values were significantly higher than the normal group PFD set of static and dynamic phase of the bladder neck, cervix and anorectal connections are significantly focused on the normal group, P<0.05. Conclusion PFD patients have different MRI images in the pelvic floor anatomy with normal female. In pelvic static and dynamic magnetic resonance PFD has important applications in the diagnosis and assessment.