Objective This paper is to discuss clinically diagnostic value and significance of 64-slice CT on ischemic lesions of intestinal wall. Methods CT diagnosis results and clinical data of thirty-two patients with ischemic lesions of intestinal wall admitted by Inpatient Department and Outpatient Department from November, 2013 to November, 2014 were retrospectively analyzed, and they were compared to results of clinically histopathologic examination to determine diagnostic value of CT. Results The number of patients suffering from the stenosis of inferior mesenteric artery by clinically pathological diagnosis accounted for 50.00%, and the number of patients suffering from superior and inferior artery of mesenterium occupied 31.25%, and the number of patients suffering from abdominal aortic stenosis hit 18.75%, 64-slice CT diagnosis for abovementioned diseases was 46.88%, 28.12% and 21.88% respectively. According to clinically pathological diagnosis, the sensitivity, accuracy and specificity of 64-slice CT hit 90.63%, 96.88% and 87.5% respectively. There was not obvious difference in the comparison between accuracy and clinical pathological diagnosis. The comparison between sensitivity & specificity and clinically pathological diagnosis satisfied X squared X2 = 9.37 and 12.70 respectively (P < 0.05), and the difference was statistically significant. Conclusions 64-slice CT delivers high clinically diagnostic rate on ischemic lesions of intestinal wall, and it is noninvasive, timely and highly safe, imposing highly clinical use value and practical significance.