Objective To investigate the diagnostic value of multi-slice spiral CT (MSCT) combined with detection of biochemical indicator of serum amylase (S-Amy), urinary amylase (U-Amy) and pancreatic lipase (LPS) for acute pancreatitis. Methods 82 patients with acute pancreatitis who were treated in our hospital from January 2016 to August 2018 were included in the study.Clinical and related imaging data were collected to analyze the imaging characteristics of acute pancreatitis and the diagnostic sensitivity and specificity of different examination method. Results The sensitivity and specificity of MSCT combined with S-Amy, U-Amy and LPS for the diagnosis of acute pancreatitis were 94.6% and 93.2%, respectively, which were significantly higher than those of the MSCT, S-Amy, U-Amy and LPS alone, and were significantly higher than those of double and triple combination examination of MSCT, S-Amy, U-Amy and LPS(P<0.05).MSCT scan of acute edematous pancreatitis showed that the volume of pancreas was normal, the contour of the pancreas was clear or blurred, there was no significant change or slight decrease in density of pancreas, and peripancreatic effusion and thickening of prerenal fascia partially were seen. Enhanced scan showed uniform enhancement of the lesion. The volume of pancreas in the scan of acute necrotizing pancreatitis can be significantly increased. And it appears as scattered, spotted or large low-density lesions. It also showed area with reduction of density was mingled with shadow of increasing density, with blurred contour. Further enhanced scanning showed uneven enhancement, the necrotic area was not strengthened, and the normal tissue was strengthened. Conclusion Joint examination of MSCT,S-Amy, U-Amy and LPS has higher sensitivity and specificity for the diagnosis of acute pancreatitis, and can clearly show the tissue of pancreas, which is beneficial to clinical qualitative diagnosis.